Glycolate excretion by Rhodospirillum rubrum |
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Authors: | Ivar Storrø A. McFadden |
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Affiliation: | (1) Institute of Biological Chemistry and Biochemistry/Biophysics Program, Washington State University, 99164 Pullman, Washington, USA |
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Abstract: | Glycolate can be measured in the supernatant fraction after incubation of butyrate-grown cells of Rhodospirillum rubrum either colorimetrically by the Calkins method or enzymatically using glycolate oxidase. Under optimal conditions, half-maximal excretion occurs at 11% O2 and the maximal rate is 6.9 nmol of glycolate min-1 mg protein-1 at 30°C. The pH and temperature optima are 7.6 and 30°C and light intensity is saturating in the range of 2–10×104 lux. Carbon dioxide inhibits glycolate excretion and exogenous butyrate stimulates. Glycolate excretion is maximal by butyrate-light grown cells harvested in the early stationary phase and under all conditions is proportional to the cellular content of ribulose 1,5-bisphosphate carboxylase/oxygenase.Non-Standard Abbreviations Bicine (N,N-bis[2-hydroxyethyl]glycine) - RuBP d-ribulose-1,5-bisphosphate - HPMS 2-pyridylhydroxymethanesulfonate |
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Keywords: | Glycolate excretion Rhodospirillum rubrum Rihulose bisphosphate carboxylase/oxygenase Photorespiration Photosynthesis |
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