ULTRAVIOLET B IRRADIATION MODULATES SUSCEPTIBILITY TO TUMOUR NECROSIS FACTOR-α-INDUCED APOPTOSIS VIA INDUCTION OF DEATH RECEPTORS IN MURINE FIBROBLASTS |
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| Authors: | Hirokazu Kimura Hisanori Minakami Akira Shoji |
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| Affiliation: | a Department of Biological Sciences, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515;b Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052;c Department of Reproductive and Developmental Medicine, Hokkaido University Graduate School of Medicine, Sapporo, 060-8638, Japan |
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| Abstract: | Ultraviolet B (UVB) irradiation causes cell death by apoptosis in murine fibroblast cells. Tumor necrosis factor-α (TNF-α) is also a well known inducer of apoptosis, although the physiological significance of this activity is poorly understood. We investigated the effects of pretreatment with UVB (312 nm) on TNF-α-induced apoptosis in murine fibroblast cells. UVB enhanced susceptibility to cell death by TNF-α in a dose-dependent manner. UVB but not TNF-α induced the expression of TNF receptor type-1 (TNFR-1) and type-2 (TNFR-2) in a dose-dependent manner. Expression of Fas (CD95) and Fas-ligand (Fas-L), and significant DNA fragmentation were observed in the cells that died. These results suggest that UVB irradiation modulates susceptibility to TNF-α-induced apoptosis through the induction of TNFRs, Fas, and Fas-L in murine fibroblasts. |
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| Keywords: | apoptosis fibroblast TNF-α TNF receptor ultraviolet |
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