Obtaining immunoglobulin-binding sorbent by immobilization of recombinant human proinsulin protein A domain fusion protein |
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| Authors: | S. N. Eremenko D. M. Koulich V. E. Petukhov K. V. Maltsev A. N. Wulfson A. I. Miroshnikov |
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| Affiliation: | (1) Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Mikluho-Maklaya 16/10, GSP V-437 Moscow, Russia |
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| Abstract: | Summary Recombinant human proinsulin - protein A domain fusion protein (rPFP) was immobilized on synthetic matrix, resulting in inexpensive immunoglobulin-binding sorbent. This is an alternate to a protein A affinity support. A Standard technique was used for immobilization. Experiments, optimizing in ligand concentration, were performed. |
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