A chemotactic factor for rat thymocytes may regulate T-lymphocyte migration toward the thymic microenvironment |
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| Authors: | A Imaizumi M Torisu T Yoshida |
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| Institution: | 1. Division of Clinical Immunology, First Department of Surgery, School of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan;2. Laboratory of Basic Toxicology, Development Research Laboratory of Banyu Pharmaceutical Company, Ltd., 810 Menuma-machi, Ohsato-gun, Saitama 360-02, Japan;3. Research Laboratories, Chugai Pharmaceutical Company, Ltd., 3-41-8 Takada, Toshima-ku, Tokyo 171, Japan;1. Department of Cell and Molecular Biology, Sanjeev Biomedical Research Centre, 1/15, Kumaran Nagar, Keelkattalai, Chennai, 600117, Tamilnadu, India;2. State Licensing Authority, Directorate of Indian Medicine (Govt. of Tamilnadu), State Licensing Authority (IM), Chennai, 600106, Tamilnadu, India;3. Department of Physics, University College of Engineering BIT Campus, Anna University, Tiruchirappalli, 620 024, Tamilnadu, India;4. Head, Research & Development, Sanjeev Biomedical Research Centre, 1/15, Kumaran Nagar, Keelkattalai, Chennai, 600117, Tamilnadu, India;1. Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Poleczki 19, 02-822 Warsaw, Poland;2. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland;3. Institute of Physics, Polish Academy of Sciences, al. Lotników 32/46, 02-668 Warsaw, Poland;1. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Environmental and Life Sciences, Wrocław, Poland;2. Department of Animal Products Technology and Quality Management, Faculty of Biotechnology and Food Sciences, Wrocław University of Environmental and Life Sciences, Wrocław, Poland;3. Biology Department, School of Science and Technology, Nazarbayev University, Kazakhstan;4. Laboratory of Immunobiology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland |
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| Abstract: | Using a modified Boyden chamber assay, extracts or culture supernatants of rat thymic stromal cells, or thymocytes were examined by chemotactic activity to rat leukocytes. Rat thymocytes responded chemotactically to the aqueous extract as well as to culture supernatants of thymic stromal cells. However, neither the extract and culture medium from concanavalin A-stimulated thymocytes nor any component of rat serum has shown such an activity. The thymic extract was fractionated into three molecular species with chemotactic activity for thymocytes. The thymocyte chemotactic factor(s) (TCFs) in the extract was distinct from known lymphocytic chemotactic factors, such as interleukin-1 (IL-1), IL-2, C5a, and the culture supernatant of stimulated thymocytes. In vitro, TCFs could attract, in addition to thymocytes, bone marrow cells, fetal liver cells, and nylon-wool nonadherent lymphocytes from peripheral blood and spleen. Lymph node cells, neutrophils, macrophages, and B cells from peripheral blood could not respond to TCFs. Thymocytes also responded to the extract of splenic stromal cells. Unlike the thymic extract, however, the splenic extract was chemotactically active for lymphocytes from lymph nodes but not for bone marrow cells. These results indicate that thymic stromal cells secrete a chemotactic factor(s) for a relatively immature type of T-lineage cells, which may by a thymus-homing progenitor T cell, while spleen may contain an attractant for a relatively mature type of T-lineage cells. |
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