不同衰退等级蒙古沙冬青根内真菌多样性与群落结构

本研究根据沙冬青现有的生长状况,将内蒙古西鄂尔多斯保护区珍稀濒危植物蒙古沙冬青群落分为轻度衰退、中度衰退和重度衰退3个衰退等级,并选取一个未衰退的沙冬青群落作为对照,应用Illumina Hiseq测序平台,对这4个沙冬青群落的植株根内真菌进行高通量测序分析。测序共获得215个真菌的OTU,不同衰退等级沙冬青根内真菌群落结构在门、科、属水平上都有显著差异。Top 10属中的Agaricus、Tomentella、Tricholoma、Fusarium、Inocybe以及Tuber这6个属真菌在所有衰退等级的沙冬青都有分布。不同衰退等级的沙冬青根内都有腐生或寄生真菌和“共生”真菌分布,不同衰退等级两类群的真菌占比不同且呈现出动态变化,随着衰退等级的增加沙冬青根内腐生真菌或寄生真菌与“共生”真菌比例显著增高。土壤有机质与土壤容重具有协同作用,且对Agaricus、Inocybe、Fusarium、Penicillium、Amphinema具有正相关影响,对Tricholoma、Tomentella、Tuber具有负相关影响。

Diversity and community structure of entophytic fungi in roots of Ammopiptanthus mongolicus in different recession degrees

According to current growing condition, endangered plant Ammopiptanthus mongolicus communities in West Erdos National Nature Reserve of Inner Mongolia were divided into three recession degrees, mild recession, moderate recession and serious recession. Unrecession community was set up as control (CK). Entophytic fungi in roots of A. mongolicus from different communities were analyzed applying Illumina Hiseq platform of high throughput sequencing. 215 OTU have been obtained, showing significant difference at the levels of phylum, family, and genus. Fungi belonging to Agaricus, Tomentella, Tricholoma, Fusarium, Inocybe and Tuber were shared in all roots tested. Saprophytic or parasitic and mutualistic fungi distribute in all A. mogolicus communities with different recession degrees, but their magnitude proportions are different and show dynamic changes. The proportion of saprophytic or parasitic fungi to mutualistic fungi increased with the increase of recession degree. Soil organic materials and bulk density have synergistic effect on the community structure of endophytic fungi in roots of A. mongolicus, showing positive effect on fungi belonging to the genera of Agaricus, Inocybe, Fusarium, Penicillium, and Amphinema, and negative effect on fungi belonging to genus of Tricholoma, Tomentella, Tuber.