牛樟芝子实体醇提物对过敏性肺炎的作用

本文研究台湾特有牛樟芝子实体醇提物对过敏性肺炎的作用并探讨其可能的作用机制。体内实验采用卵清蛋白（OVA）致敏制备过敏性肺炎模型,观察各组肺组织病理变化情况,并对脂多糖（LPS）诱导的细胞分泌促炎性细胞因子进行检测;离体实验中采用酶联免疫吸附法（ELISA）对LPS刺激THP-1细胞分泌促炎性因子（IL-1、IL-6及TNF-α）进行了检测。体外巨噬细胞（THP-1）抗炎试验结果显示牛樟芝子实体醇提物不同浓度均可显著抑制LPS诱导的THP-1细胞IL-1的分泌（P<0.001）;可完全抑制IL-6的分泌;浓度20μg/mL及80μg/mL下可以完全抑制TNF-α的分泌。OVA过敏性肺炎动物模型中,与模型组相比,牛樟芝子实体醇提物组可显著改善肺部外观色泽、细支气管淋巴球聚集、肺泡壁增生及肺泡空腔等炎症程度。口服KBA皿式牛樟芝子实体醇提物可有效改善过敏性肺炎程度,并有全身系统性抗炎效果,其机制可能为抑制THP-1细胞IL-1、IL-6及TNF-α的分泌。本研究为深入研究KBA皿式牛樟芝子实体改善雾霾性肺炎提供了重要的实验依据。

Effects of ethanol extract of Taiwanofungus camphoratus fruiting body on the OVA-induced allergic pneumonia

To study effects of ethanol extract (EE) of Taiwanofungus camphoratus fruiting body (TCF) endemic to Taiwan on the ovalbumin (OVA)-induced allergic pneumonia and explore its possible mechanism. In vivo experiments using ovalbumin (OVA) sensitized to prepare allergic pneumonia model, observed the pathological changes of lung tissue in each group, and detected secretion of proinflammatory cytokines in cells induced by lipopolysaccharide (LPS); in vitro experiments using enzyme-linked immunosorbent assay (ELISA) determined the secretion of pro-inflammatory cytokines (IL-1, IL-6 and TNF-α) from THP-1 cells stimulated by LPS. In vitro macrophage (THP-1) anti-inflammatory test results showed that different concentrations of TCFEE could significantly inhibit LPS-induced THP-1 cell IL-1 secretion (P<0.001), completely inhibit the secretion of IL-6 and TNF-α secretion at concentrations of 20 and 80μg/mL. In the animal model of OVA-induced allergic pneumonia, compared with the model group, the KBA-TCFEE can significantly improve the appearance of lung color, bronchiolar lymphocyte aggregation, alveolar wall proliferation and alveolar cavity and other inflammation. Our results inferred that oral KBA-TCFEE effectively improved the OVA-induced allergic pneumonia and exhibited the systemic anti-inflammatory effect. The mechanism may be to inhibit the secretion of IL-1, IL-6 and TNF-α in THP-1 cells. The results can be also used as an important reference to prevent and control the harmful effects of haze pneumonia.