仓储烟叶表面真菌群落组成分析

利用高通量测序平台,对贮存于贵阳库（GY）、坛厂库（TC）和紫云库（ZY）的云南保山C3F（2013）烟叶样品进行宏基因组水平的ITS1基因测序分析,以期揭示陈化烟叶表面真菌群落组成、分布格局及功能分组,探讨化学因子对其结构的影响。结果显示,从15个样品中共检测到了1 173 560条有效序列,包括4个门、140个属;贵阳库优势属为耐干霉菌属Xeromyces,坛厂库优势属为红酵母属Rhodotorula,紫云库优势属为耐干霉菌属Xeromyces;坛厂库优势菌群及变化趋势与贵阳库和紫云库存在差异;真菌群落以腐生营养型和病理营养型为主,分布有潜在人体病原真菌;有机碳、总磷、总钾对真菌群落变化影响显著（P<0.05）。真菌群落组成和格局分布受存储环境、陈化时间及烟叶化学元素的共同影响。

Analysis of fungal community composition on the stored tobacco surfaces

ITS1 genes of the fungi isolated from the stored tobacco sample C3F (2013) from Baoshan City, Yunnan Province, stored in Guiyang storeroom (GY), Tanchang storeroom (TC) and Ziyun storeroom (ZY), were sequenced using Illumina HiSeq2500 high-throughput sequencing platform. The composition, distribution and functional group of fungal communities were studied, and the influence of chemical factors on community structure was discussed. The results showed that 1 173 560 valid reads from 15 samples were obtained, including four phyla and 140 genera of different fungi. The dominant genera in GY, TC and ZY were Xeromyces, Rhodotorula and Xeromyces, respectively. The dominant community and changed trend in TC were significantly different from those in GY and ZY. Nourishment types of tobacco-inhabiting fungi were dominantly saprotrophic and pathotrophic, hiding potential human fungal pathogens. The effect of organic carbon, total phosphorus and total potassium on fungal community variation was significant (P<0.05). The composition and distribution of fungal communities were influenced by storage environment, aging time and chemical factors of tobacco leaves.