禾谷镰孢菌蛋白激酶FgCdc15功能的研究

细胞分裂是真核细胞生长发育的重要环节。本研究利用生物信息学的方法,通过使用酵母菌Cdc15蛋白激酶序列比对,发现禾谷镰孢菌中只存在一个Cdc15蛋白激酶。基因缺失的功能研究表明FgCDC15（FGSG_10381）基因敲除后,突变体菌落生长速度减慢,对小麦和玉米无致病力。FgCDC15基因敲除突变体产生的分生孢子外观形态正常,但隔膜数量变少,同时发现该基因在分生孢子阶段表达量最高。在有性生殖阶段,FgCDC15基因敲除突变体可产生极少量的子囊壳,但不产生子囊和子囊孢子。本文研究表明,禾谷镰孢菌蛋白激酶FgCdc15可能参与了有性和无性阶段的细胞分裂和生长,同时影响禾谷镰刀菌的致病毒力。

Functional characterization of protein kinase FgCdc15 in Fusarium graminearum

Cell division is an important cellular process in eukaryotic cells. Protein kinase Cdc15 is associated with cell division in budding yeast. In this study, the conserved ortholog of the yeast Cdc15 protein kinase was predicted in Fusarium gramniearum as a single copy. Loss-of-function of this kinase gene was performed and the results showed that deletion of FgCDC15 (FGSG_10381) resulted in a decreased growth rate and loss of virulence against wheat and maize as compared with the wild-type strain. Except for the normal conidial phenotype, the number of septa was reduced in the mutants, and the expression of FgCDC15 gene was the highest in the stage of conidiogenesis. During sexual reproduction, the mutants produced fewer perithecia than wild-type strain and did not produce asci and ascospores. It is concluded that the protein FgCdc15 might be involved in cell division, sexual and asexual development, and regulation of the infection process of F. graminearum.