冠突曲霉 veA基因缺失型与野生型的差异代谢物研究

为了鉴定冠突曲霉 veA基因缺失型与野生型的差异代谢物,寻找与 veA 基因产孢相关的代谢物,采用气相色谱-质谱联用（GC-MS）的代谢组学技术,分别收集2个菌株培养48h的菌丝体,经液氮研磨、超声萃取、三甲基氯硅烷衍生化后上机检测,将GC-MS检测的数据进行前处理和代谢物注释,并用统计学相关软件进行差异物筛选和相关性分析。结果显示,共鉴定99种代谢物,其中差异代谢物41种,野生型菌株中表达上调的显著差异代谢物有20种, veA缺失型菌株中表达上调的显著差异代谢物有21种,并预测差异代谢物的相关性,发现与623种代谢物产生相关性,其中313种呈正相关,310种呈负相关。筛选出来的差异代谢物涉及有机酸、氨基酸、碳水化合物、醇类、脂肪酸类等多种代谢物质,其中有机酸和氨基酸类代谢物占主导地位。本研究结果为进一步研究冠突曲霉代谢物与产孢的关联提供了一定的理论基础。

Differential metabolite analysis of the veA gene deletion and wild type strains of Aspergillus cristatus

The metabolites of mycelial samples of ΔveA mutant and wild-type strain E4 of Aspergillus cristatus with 48 hour incubation period were identified by gas chromatography-mass spectrometry (GC-MS). A total of 99 metabolites were identified, including 41 significantly differential metabolites. Among them, 20 and 21 metabolites were much increased in the wild-type E4 and ΔveA, respectively. Statistical analysis revealed there were 623 individual metabolite having correlation, of which 313 were positive and 310 negative. These metabolites include organic acids, amino acids, carbohydrates, alcohols and oxylipin, while organic acids and amino acids predominate. This result lays the foundation for further study on the correlation between metabolite and sporulation in A. cristatus.