ARTP诱变猴头菌株的发酵菌丝体多糖理化性质及体外免疫活性

为探讨常压室温等离子体诱变的3株高产多糖猴头菌和出发菌株的多糖组分差异,通过液体发酵获得的菌丝体经水提、分级醇沉获得8个胞内多糖组分,对它们的理化性质、结构特征及体外免疫活性进行了研究。结果表明,3株ARTP诱变菌株414、321、236菌丝体多糖含量较出发菌株有较明显提升;ARTP诱变的猴头菌20%醇沉多糖组分较出发菌株分子量大,所占比例增加;诱变菌株60%醇沉多糖组分的分子量略大于出发菌株,所占比例相近。20%醇沉多糖主要由半乳糖、葡萄糖、甘露糖构成,诱变菌株该多糖组分中葡萄糖和甘露糖的比例较出发菌株均有明显提升,60%醇沉多糖组分单糖组成无明显差异;8个多糖组分均具有体外刺激巨噬细胞释放NO的活性,其中20%醇沉多糖的活性优于60%醇沉多糖,诱变菌株的生物活性优于出发菌株。本研究探讨了ARTP诱变对猴头菌胞内多糖结构及活性的影响,为猴头菌相关产品的开发提供了优质资源。

Physicochemical properties and immunological activities in vitro of mycelial polysaccharides from Hericium erinaceus mutants induced by atmospheric and room temperature plasma

The proportional differences between polysaccharides extracted from ARTP mutagenic strains and those from strain of Hericium erinaceus were compared. The polysaccharide of mycelia obtained from liquid fermentation were extracted with boiling water and graded by ethanol precipitation. Eight crude intracellular polysaccharide fractions were obtained and their physicochemical properties, structural characteristics and in vitro immunological activity were studied. The results indicated that polysaccharide content of mycelia of mutants 414, 321, 236 was significantly higher than that of the original strain. The proportion of ethanol-precipitated polysaccharides of H. erinaceus mutagenic strains was significantly higher than that of the ten million-level of high-molecular-weight polysaccharides of the original strain, and the proportion of polysaccharides with a million-level molecular weight was relatively close. The 20% ethanol-precipitated polysaccharide fraction of the mutants had higher molecular weight than that of the original strain, and the proportion of polysaccharides increased. The molecular weight of the 60% ethanol-precipitated polysaccharide fractions of the mutants was slightly larger than that of the original strain, and the proportion was similar. 20% ethanol-precipitated polysaccharides mainly consist of galactose, glucose, and mannose. The proportion of glucose and mannose in the polysaccharide components of the mutants was significantly higher than that of the original strain, and there was no significant difference in the monosaccharide composition of the 60% ethanol-precipitated polysaccharides. All eight polysaccharide fractions had the activity of stimulating NO release from macrophages in vitro. The activity of 20% ethanol-precipitated polysaccharides was better than that of 60% ethanol-precipitated polysaccharides and showed significant immunological activity in vitro. The biological activity of mutagenized strains was better than that of the original strain. This study might provide a reference for obtaining high quality resources of H. erinaceus and developing related products.