太行山片麻岩区栓皮栎外生菌根真菌多样性

以太行山片麻岩区栓皮栎Quercus variabilis为研究对象,利用形态学、常规测序与Illumina MiSeq高通量测序相结合的方法,调查并鉴定了与其共生的外生菌根真菌。最终共划分出18种栓皮栎外生菌根形态类型。高通量测序得到394个外生菌根真菌OTUs（operational taxonomic units）,隶属于11目23科30属。其中栓皮栎外生菌根核心菌群（core microbiome）共9属17个OTUs,总相对多度达65.16%。样地1与样地2中样本的多样性指数无明显差异,但非度量多维尺度分析（NMDS）表明核心菌群在2个样地中的分布不同,其中红菇属Russula、牛肝菌属Boletus、粉孢牛肝菌属Tylopilus、硬皮地星属Astraeus主要分布于位于阳坡的样地1,而空团菌属Cenococcum、小球孢盘菌属Sphaerosporella、锁瑚菌属Clavulina、丝盖伞属Inocybe主要分布于位于阴坡的样地2,毛革菌属Tomentella在两个样地的分布相似。

Diversity of ectomycorrhizal fungi associated with Quercus variabilis in gneissose area of Taihang Mountains

The diversity of ectomycorrhizal fungi associated with Quercus variabilis in gneissose area of Taihang Mountains was investigated by using the combination of morphological method, Sanger sequencing and Illumina MiSeq sequencing platform. A total of 18 morphological types of ectomycorrhizae on Q. variabilis were identified. A total of 394 operational taxonomic units (OTUs) of ectomycorrhizal fungi were obtained using Illumina MiSeq sequencing techniques, belonging to 11 orders, 23 families, 30 genera, of which 17 OTUs of nine genera were defined as core microbiome of ectomycorrhizal fungi on Q. variabilis, and the total relative abundance of them was 65.16%. There was no significant difference between two plots in diversity indices. However, the results of the NMDS (nonmetric multidimensional scaling) analysis showed that the abundance of core microbiome was different between plot 1 and plot 2. Russula, Boletus, Tylopilus and Astraeus were mainly distributed at plot 1 which was located in sunny slope. Cenococcum, Sphaerosporella, Clavulina and Inocybe mainly occurred in the plot 2 which was located in shady slope. The distribution of Tomentella was fairly similar between plot 1 and plot 2.