以松毛虫为基质培养的冬虫夏草菌丝体中多糖的提取研究

研究了不同方法对冬虫夏草发酵菌丝体（以枯叶蛾科昆虫马尾松毛虫为基质发酵所得）中多糖提取的影响。结果表明,采用微波辅助水提法所得多糖的产率最高,影响提取的关键因素为液料比、微波提取时间、微波功率;采用Box-Behnken设计及响应面分析法对这3个因素进行优化,并通过回归拟合,建立了预测虫草多糖提取的多项式模型Y=6.87+0.058A+0.085B+0.075C+0.032AB+0.046AC+0.069BC-0.16A²-0.37B²-0.11C²。经响应面最优化分析,获得冬虫夏草发酵菌丝体中多糖的最优提取工艺参数为：液料比（mL/g）6.3:1、微波功率520W、微波提取时间326s,此工艺提取验证后的提取率达到6.76%。

Extraction technology of polysaccharides of Ophiocordyceps sinensis mycelium cultured in medium using the insect Dendrolimus punctatus as substrate

The effects of different extraction methods on the extraction of polysaccharide from Ophiocordyceps sinensis mycelium (PDOM) fermented in medium using the insect Dendrolimus punctatus (Lasiocampidae) as substrate were comparatively investigated. The results showed that the optimal extraction method was the application of microwave-assisted extraction technique, and the key factors influencing the extraction were the ratio of water to mycelium, microwave extraction period and microwave power. Box-Behnken test design combined with response surface methodology were applied to optimize the extraction process of PDOM. The polynomial model Y=6.87+0.058A+0.085B+0.075C+0.032AB+0.046AC+0.069BC-0.16A²- 0.37B²-0.11C² is established to predict the extraction of PDOM. It was showed that the optimal process parameters were the ratio of water to mycelium (V/M, mL/g) 6.3:1, microwave power 520W, and microwave extraction period 326s. Under such a process the extraction rate of PDOM reached 6.76%.