刺芹侧耳栽培料及菌体中漆酶介体分子的挖掘

虽然真菌能够分泌漆酶从木质素中获取碳源,然而纯化后真菌漆酶降解木质素的能力却极低。本文利用萃取方法提取刺芹侧耳栽培料及菌体中可溶性化合物,通过快速漆酶介体体系（LMS）动力学实验确定其对漆酶催化氧化反应的促进或抑制能力,进一步利用HPLC-MS和代谢组学差异分析的方法从最高活性萃取液的柱层析组分中挖掘潜在的活性成分。研究结果表明栽培料中可能含有活性介体成分,生物信息学XCMS代谢产物组分析揭示了CTE05分离组分中富含的木质素代谢产物,涉及到软木脂单体合成、香豆素合成、丁香油酚或异丁香油酚合成、质体醌醇合成、由苯丙氨酸合成硫代葡萄糖苷和安息香酸合成等代谢途径。最后讨论了其中11种潜在的天然介体分子物理化学性质,其中4种为已有文献报道的天然介体小分子。本研究暗示木质素降解与合成代谢在分子机制上存在有某种联系,相关的代谢产物可能参与了真菌降解木质素的代谢过程。

Exploration of laccase-natural mediator from the compost and fruiting bodies of Pleurotus eryngii

Although in nature fungi secrete laccases to digest lignin for source of carbon, practically the artificially purified laccase shows extremely low activity. In this study soluble compounds from cultivating compost, mycelia, and fruiting bodies of Pleurotus eryngii were extracted, and their regulation degree (RD%) for laccase-catalyzed reaction was measured. Potential mediator molecules from the most active components were searched by using HPLC-MS and metabolomics methods. It was found that the toluene-extract of compost (CTE) possessed the active mediators, and further comparative analysis revealed that the most active component CTE05 were rich in lignin-related metabolites involving the biosyntheses of suberin monomer, coumarin, eugenol-or-isoeugenol, plastoquinol-9, glucosinolate (from phenylalanine), and benzoate. The 11 potential mediator molecules were discussed in terms of similarity in physicochemical properties, including four ones which have been validated in previous literatures. Our study suggested that lignin biodegradation and biosynthesis might be related to each other in metabolism, and lignin-related metabolites might take part in fungal lignin digestion.