灰树花孔菌超微粉体内抗肿瘤作用研究

通过研究肿瘤抑制率、脏器指数、肿瘤切片的观察以及血清中影响因子干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）、血管内皮细胞生长因子（VEGF）、过氧化氢酶（CAT）、谷胱甘肽-过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）等指标的变化对比分析灰树花孔菌 Grifola frondosa子实体粗粉及灰树花孔菌子实体超微粉对Hep-A-22荷瘤小鼠体内抗肿瘤和免疫调节作用。结果表明,各剂量组分均具有抑制肿瘤生长作用,其中灰树花孔菌超微粉高剂量组（1 500mg/kg）抑瘤效果最佳,与模型组比较有极显著性差异（ P<0.01）。通过HE染色后的肿瘤细胞可观察到坏死面积增大,同时该组血清中IFN-γ、IL-2、VEGF、GSH-PX和SOD含量显著增加（ P<0.01或 P<0.05）,并与抑制肿瘤具有一定相关性。

Anti-tumor activities of Grifola frondosa submicron powder on tumor- bearing mice

Determination of tumor inhibition rate and spleen and thymus index, and observation of tumor slices and the changes of interferon-γ (IFN-γ), interleukin-2 (IL-2), vascular endothelial growth factor (VEGF), catalase (CAT), glutathione-peroxidase (GSH-PX) and superoxide dismutase (SOD) in serum were carried out to analyze the anti-tumor activities and immune regulation effects of Grifola frondosa fruiting body coarse powder and submicron powder on Hep-A-22 tumor-bearing mice. The results showed that the anti-tumor remedy of high dose submicron powder (1 500mg/kg) experimental group was of the best effect showing significant difference ( P<0.01) as compared with the model experimental group. Increased necrotic area of tumor was observed by HE staining. The content of IFN-γ, IL-2, VEGF, GSH-PX and SOD in serum of the treated group were significantly increased, showing a correlation with the tumor inhibition rate.