Multiparametric comparison of mesenchymal stromal cells obtained from trabecular bone by using a novel isolation method with those obtained by iliac crest aspiration from the same subjects |
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Authors: | Fermin M Sanchez-Guijo Juan F Blanco Graciela Cruz Sandra Muntion Maria Gomez Soraya Carrancio Olga Lopez-Villar Maria-Victoria Barbado Luis-Ignacio Sanchez-Abarca Belén Blanco Jesus G Briñon Maria-Consuelo del Cañizo |
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Institution: | (1) Servicio de Hematología, Hospital Universitario de Salamanca, Salamanca, Spain;(2) Servicio de Traumatología, Hospital Universitario de Salamanca, Paseo de San Vicente 58–182, 37007 Salamanca, Spain;(3) Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Salamanca, Spain;(4) Departamento de Biología Celular y Patología, Universidad de Salamanca, Salamanca, Spain |
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Abstract: | Trabecular bone fragments from femoral heads are sometimes used as bone grafts and have been described as a source of mesenchymal
progenitor cells. Nevertheless, mesenchymal stromal cells (MSC) from trabecular bone have not been directly compared with
MSC obtained under standard conditions from iliac crest aspiration of the same patients. This is the ideal control to avoid
inter-individual variation. We have obtained MSC by a novel method (grinding bone fragments with a bone mill without enzymatic
digestion) from the femoral heads of 11 patients undergoing hip replacement surgery and compared them with MSC obtained by
standard iliac crest aspiration of bone marrow from the same patients. We have shown that trabecular bone MSC obtained by
mechanically fragmented femoral heads fulfil the immunophenotypic and multilineage (adipogenic, osteogenic and chondrogenic)
differentiation criteria used to define MSC. We have also differentially compared cellular yields, growth kinetics, cell cycle
assessment, and colony-forming unit-fibroblast content of MSC from both sources and conclude that these parameters do not
significantly differ. Nevertheless, the finding of slight differences, such as a higher expression of the immature marker
CD90, a lower expansion time through the different passages, and a higher percentage of cycling cells in the trabecular bone
MSC, warrants further studies with the isolation method proposed here in order to gain further knowledge of the status of
MSC in this setting.
The present study was partially supported by grant HUS01B07 from the Consejería de Educación and by grant SAN196/SA13/07 from
the Consejería de Sanidad, Junta de Castilla y León, Spain. |
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Keywords: | Mesenchymal stromal cells Femoral heads Bone marrow Cell culture Human |
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