A new sensitive and fast peptide immunoassay based on enzyme amplification used in the determination of CGRP and the demonstration of its presence in the thyroid |
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| Authors: | C H Self S J Wimalawansa A Johannsson D L Bates S I Girgis I Macintyre |
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| Affiliation: | 1. Department of Chemical Pathology, Royal Postgraduate Medical School, Hammersmith Hospital DuCane Road, London W12 OHS UK;1. IQ (Bio) Ltd., Downham House, Downhams Lane, Milton Road, Cambridge CB4 1XG UK |
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| Abstract: | An enzyme amplified immunoassay for rCGRP based on cofactor cycling has been found to be clearly superior to a comparable radioimmunoassay employing the same antiserum in terms of sensitivity, speed and convenience. Correlation between the two methods was very good. With the enzyme amplified immunoassay we have been able to demonstrate the existence of rCGRP in thyroid extract. |
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| Keywords: | Enzyme amplification Enzyme immunoassay Calcitonin gene-related peptide |
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