Reactions of the intracellular NADpool in the yeastS. cerevisiae after UV-C- or X-ray irradiation |
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Authors: | K. Winckler E. Herfurth |
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Affiliation: | (1) Institut für Biophysik, Freie Universität Berlin, Thielallee 63, D-1000 Berlin 33, Germany |
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Abstract: | Summary The reaction of the intracellular NADpool after irradiation of cells either with UV-C light or with X-rays was studied in four different strains of the yeastS. cerevisiae. We found neither in wildtype strains nor in radiation sensitive mutants remarkable changes in the NADpool within 2 h after irradiation. Preculture of cells in medium enriched with nicotinic acid, a precursor of NAD, influenced the intracellular NAD concentration only to a small extend in all strains, but enhanced the radiation resistance against UV-C significantly in one rad6 mutant strain. The uptake of NAD and NAC by all strains before and after irradiation with UV-C and X-ray was tested also. NAD generally is taken up by the cells to a very low extent before and after irradiation without irradiation-dose dependency. NAC is taken up by all strains before and after irradiation. Only the rad6 mutant exhibited an irradiation-dose dependent NAC-uptake after UV-C irradiation.Abbreviations ADPRT ADPribosyltransferase - NAC nicotinic acid - YEPG yeast extract-peptone-glucose medium |
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