The SCID/Beige mouse as a model to investigate protection against Yersinia pestis |
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Authors: | Green M Rogers D Russell P Stagg A J Bell D L Eley S M Titball R W Williamson E D |
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Institution: | DERA, Chemical and Biological Defense Sector, Salisbury, Wiltshire, UK. |
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Abstract: | In this study, we have shown that severe combined immunodeficient/beige mice reconstituted with hyperimmune Balb/c lymphocytes can be used as a model to demonstrate adoptive and passive protection against plague infection. Reconstitution of severe combined immunodeficient/beige mice was successful in nine out of ten mice as demonstrated by spleen colonisation and sustained circulating immunoglobulin titres. Furthermore, an increase in antibody titre was evident after a booster immunisation of reconstituted mice. Presence of circulating antibody correlated with protection against a systemic plague challenge and indicated that in reconstituted mice adoptive transfer of a functional immune system had occurred. The severe combined immunodeficient/beige mouse was also used to demonstrate passive protection against inhaled and systemic plague infection. The reconstituted severe combined immunodeficient/beige mouse model demonstrating protective immunity against plague will be further developed to identify the immune cell subsets responsible for this protection. |
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Keywords: | Severe combined immunodeficient/beige mouse Plague Adoptive protection Passive protection |
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