Establishment of a human anaplastic thyroid cancer cell line secreting granulocyte colony-stimulating factor in response to cytokines |
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Authors: | Yoshio Oka Tetsuro Kobayashi Shoichi Fujita Nariaki Matsuura Shigeru Okamoto Hideki Asakawa Atsuo Murata Takesada Mori |
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Affiliation: | (1) Department of Pathology II, Wakayama Medical School, Wakayama, Japan;(2) Second Department of Internal Medicine, Osaka University Medical School, Osaka, Japan;(3) Department of Pathology, Osaka Teishin Hospital, Osaka, Japan;(4) Department of Surgery II, Osaka University Medical School, 1-1-50 Fukushima, Fukushima-ku, 553 Osaka, Japan |
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Abstract: | Summary A human anaplastic thyroid cancer cell line K-119, derived from a 77-yr-old woman who had developed marked neutrophilia and underwent surgery for anaplastic thyroid cancer, has been established. The spindlelike and polygonal cells in shape are stably proliferating since the beginning of its culture 2 yr ago. The cells grow rapidly and the population doubling time is 26 h. The chromosomes show many abnormalities and many marker chromosomes have been observed. Heterotransplantation of the cells into nude mice has resulted in the formation of tumors that are histologically interpreted as anaplastic cancer. The most noteworthy characteristics of the cell line are the many Ki-67-positive cells (86.3%) and that the cell line spontaneously secretes granulocyte colony-stimulating factor (G-CSF) and releases increased amounts of G-CSF in response to the stimulation of tumor necrosis factor, interleukin 1α, and interleukin 1β. The conditioned medium obtained from K-119 cells contains an autocrine factor stimulating the proliferation of themselves. |
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Keywords: | G-CSF thyroid cancer TNF IL-1 Ki-67 autocrine |
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