Intestinal absorption of lysozyme,an egg-white allergen,in rats: Kinetics and effect of NSAIDs |
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Authors: | Tomoharu Yokooji Koh Hamura Hiroaki Matsuo |
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Affiliation: | 1. Department of Pathophysiology and Therapeutics, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;2. Faculty of Pharmaceutical Sciences, Hiroshima University, Hiroshima, Japan |
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Abstract: | The absorption pathway(s) of a representative food allergen, lysozyme, and the mechanisms of lysozyme absorption facilitated by non-steroidal anti-inflammatory drugs were examined by intestinal closed-loop and re-circulating perfusion methods in rats. The absorption rate of fluorescein isothiocyanate (FITC)-labeled lysozyme in the proximal intestine was higher than that for a marker of non-specific absorption, FD-10, and was suppressed by colchicine (endocytosis inhibitor). Aspirin increased the absorption of FITC-lysozyme in the proximal intestine with no effects on tissue accumulation. Diclofenac facilitated FITC-lysozyme absorption, but meloxicam and loxoprofen exerted no effects on absorption. Co-administration of misoprostol (synthetic prostaglandin-E1 analog) with aspirin significantly ameliorated the aspirin-facilitated absorption of FITC-lysozyme to the same level as that seen with controls. Thus, lysozyme absorption was mediated by endocytic and paracellular pathways in the proximal intestine, and was facilitated by aspirin and diclofenac after impairment of the paracellular pathway. Misoprostol may suppress the allergen absorption facilitated by aspirin. |
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Keywords: | FITC, fluorescein isothiocyanate FD, fluorescein isothiocyanate-labeled dextran FDEIA, food-dependent exercise-induced anaphylaxis Ig, immunoglobulin NSAIDs, non-steroidal anti-inflammatory drugs COX, cyclooxygenase SDS&ndash PAGE, sodium dodecyl sulfate&ndash polyacrylamide gel electrophoresis CBB, coomassie brilliant blue PBS, phosphate-buffered saline DMSO, dimethyl sulfoxide AUC, area under the plasma concentration&ndash time curve |
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