DNA methyltransferase inhibitor assay system based on the HBx-induced DNA methylation of E-cadherin |
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Authors: | Hyehyeon Lee Jin Bae SohnSoo Shin Kim Kyung Lib Jang |
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Affiliation: | Department of Microbiology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea |
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Abstract: | We here report a simple assay system for DNA methyltransferase (DNMT) inhibitors based on the HBx-induced DNA methylation of E-cadherin. A stable cell line named G1 was generated by co-transfecting E-cadherin luciferase reporter and HBx-expression plasmid into HepG2 cells. Treatment of G1 cells with DNMT inhibitors, 5-azacytidine, 5-aza-2′-deoxycytidine, and procainamaid, dose-dependently inhibited DNA methylation of E-cadherin promoter in the reporter, resulting in up-regulation of luciferase levels and its enzyme activity. Treatment with all-trans retinoic acid that is known to inhibit DNMT expression, also induced similar effects. Our system can be useful for development of epi-drugs targeting DNA methylation in malignancies. |
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Keywords: | DNA methyltransferase DNA methylation E-cadherin Epi-drug HBx |
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