Free-standing lipid films stabilized by Annexin-A5 |
| |
Authors: | Anne Simon,Cé line Gounou,Sisareuth Tan,Louis Tiefenauer,Marco Di Berardino,Alain R. Brisson |
| |
Affiliation: | 1. Université de Lyon 1, CNRS, INSA de Lyon, CPE Lyon, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR5246, F-69622, Villeurbanne, France;2. Laboratoire Chimie & Biologie des Membranes & Nanonoobjets, Université Bordeaux, CBMN, UMR 5248, Allée G. Saint Hilaire, F-33600 Pessac, France;3. Biomolecular Research, Paul Scherrer Institute (PSI), CH-5232 Villigen PSI, Switzerland;4. Leister Process Technologies (Axetris), 6056 Kägiswil, Switzerland |
| |
Abstract: | Free-standing lipid bilayers in nano- and micro-pores are interesting membrane models and attractive for biotechnological applications. We describe here the controlled preparation of proteo-lipid mono- and bilayers using the Langmuir–Schaefer transfer or Langmuir–Blodgett technique, respectively on hydrophobic and hydrophilic surfaces. We demonstrate the formation of suspended proteo-lipid layers by Transmission Electron Microscopy (TEM) and in situ Atomic Force Microscopy (AFM) imaging. Using Annexin-A5 as a membrane-associated protein, continuous proteo-lipid mono- and bilayers were formed, which span pore arrays over areas of several square-micrometers. The 2D organization of proteins associated to lipid monolayer is well preserved during the transfer process and the protein association is Ca2+-dependent and therefore reversible. The simple formation and reliable transfer of stabilized free-standing lipid films is a first crucial step to create biomimetic membranes for biotechnological applications and membrane protein research. |
| |
Keywords: | AFM, Atomic Force Microscopy TEM, Transmission Electron Microscopy AnxA5, Annexin-A5 |
本文献已被 ScienceDirect 等数据库收录! |
|