Arthropod hemocyanin structure: isolation of eight subunits in the scorpion. |
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Authors: | J Lamy J Lamy J Weill |
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Institution: | Department of Biochemistry, School of Medicine, 2 bis Boulevard Tonnellé, 37032 Tours Cedex, France |
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Abstract: | Eight immunologically pure subunits were isolated from Androctonus australis hemocyanin. Antisera specific against each of these were prepared. Two subunits associate to form a heteroöligomer which is probably one of the bridges visible in electron microscopy of the native molecule. There is no cross-reactivity between native subunits. When denatured by 7 m urea, the specific antigenic determinants disappear and a broader specificity appears. This is probably due to the unfolding of the molecule which unmasks deeply buried antigenic sites. |
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