Antibodies against the human cellular 3,3′,5-triiodo-L-thyronine-binding protein (p58) |
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Authors: | Toru Obata Takaaki Fukuda Sheue-yann Cheng |
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Institution: | National Cancer Institute, Laboratory of Molecular Biology, Bethesda, MD 20892. |
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Abstract: | High-titer antibodies against a cellular thyroid hormone-binding protein (Mr 58 000, p58) were developed by a special immunization method. To enhance immune responses, this method uses a boosting protocol in which repeated injections of small amounts of antigen are administered at 2-day intervals. Antibodies were detected 1 week after the last injection of antigen by ELISA, Western dot blotting and immunoprecipitation. The anti-p58 antibodies recognize p58 which is bound to the thyroid hormone. With the availability of anti-p58 antibodies, it has become possible to study cellular localization and function. |
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Keywords: | Immunization ELISA Western blotting Metabolic labeling Thyroid hormone T3-binding protein |
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