Endocytosis in absorptive cells of cultured human small-intestinal tissue: Effect of cytochalasin B and D |
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Authors: | J Blok B A A Scheven A A Mulder-Stapel L A Ginsel W Th Daems |
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Institution: | (1) Laboratory for Electron Microscopy, University of Leiden, Leiden, The Netherlands;(2) Laboratory for Electron Microscopy, University of Leiden, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands |
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Abstract: | Summary The effect of cytochalasin B (CB) and cytochalasin D (CD) on the endocytotic uptake of horseradish peroxidase (HRP) by intestinal absorptive cells was investigated by morphometric methods. The results showed that CD inhibited endocytosis considerably, and without any detrimental side-effects. CB had hardly any effect on the endocytosis of HRP, but caused a significant decrease in the number of apical vesicles and tubules involved in the transport of cell-coat glycoproteins from the Golgi apparatus to the brush border.Electron-microscopic autoradiographic analysis of the effect of CD showed that although endocytosis is inhibited significantly by the drug, the amount of radiolabelled cell-coat material entering the lysosome-like bodies was unaltered compared with control cultures. These observations support our hypothesis that the cell-coat glycoproteins of the absorptive cells enter the lysosome-like bodies by a crinophagic rather than by an exocytotic-endocytotic mechanism. |
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Keywords: | Cytochalasin B and D Endocytosis Intestine (human) Glycoprotein transport Lysosomal degradation |
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