The effect of pH and lysosomal extract on rat liver metallothionein (Cd-thionein) |
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| Authors: | H Meulenaar C J A van den Hamer T S G A M van den Ingh T M W Stolk |
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| Institution: | 1. Department of Veterinary Pathology, Faculty of Veterinary Medicine, State University, PO Box 80.158, 3508 TD, Utrecht, The Netherlands
2. Interuniversity Reactor Institute, Delft, The Netherlands
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| Abstract: | Was studied in vitro. The109Cd-labeled protein was isolated by gel filtration and incubated with a lysosomal extract from the same source. No degradation was seen when the pH of the incubation medium was 5 or higher, whereas the degradation of Cd-thionein was completed in 2 h at a pH of 4.5. Dissociation of Cd2+ from thionein, which takes place to a fair extent at a pH below 5, appears necessary before the enzymatic breakdown of the thionein moiety can take place. |
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