Copper-rich nucleoprotein generated by micrococcal nuclease |
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Authors: | Sara E. Bryan Leighton LeGros Jean Brown Chris Byrne Richard N. Re |
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Affiliation: | 1. Department of Biological Sciences, University of New Orleans, 70148, New Orleans, Louisiana 2. Division of Research, Alton Ochsner Medical Foundation, 1514 Jefferson Highway, 70121, New Orleans, LA
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Abstract: | Nuclei from calf thymus tissue digested with micrococcal nuclease under nonchelating conditions yielded soluble nucleoprotein enriched in copper. Following limited digestion, the ratio of μg Cu:mg DNA was inversely related either to percent solubility of chromatin or to levels of enzyme maintaining an enzyme:A 260 ratio of 0.059. The enzyme appeared to cleave preferentially regions of chromatin where copper is localized, releasing no additional metal upon further digestion. Moreover, the highest copper: DNA ratio was always associated with the least-digested sample. The distribution between copper and angiotensin II (AII) in chromatin fragments following slight nuclease digestion suggests a possible link between copper and nuclear AII binding. When nuclei are incubated with AII prior to digestion and dialysis, solubilized chromatin contained about three times more copper than buffer control. Metal profiles generated from gel (A-5 M) chromatography for these samples were distinctive: copper peaks appeared near or adjacent to linker DNA regions, and in the case of AII, coincided with fragments containing specific AII receptors; thus, there appears to be an enrichment of copper in these active nucleoprotein fragments. |
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