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Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato |
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| Authors: | J. H. Hämäläinen V. A. Sorri K. N. Watanabe C. Gebhardt J. P. T. Valkonen |
| Affiliation: | Institute of Biotechnology, Viikki Biocenter 1, P.O. Box 56, FIN-00014 University of Helsinki, Finland, FI Department of Biotechnological Science, Kinki University, Uchita, Wakayama, 649-64, Japan, JP Max-Planck-Institut für Züchtungsforscung, Carl-von-Linné-weg 10, D-50829 K?ln, Germany, DE Institute of Biotechnology, P.O. Box 56, FIN-00014 University of Helsinki, Finland, FI |
| Abstract: | The gene Ry adg that confers resistance to potato Y potyvirus (PVY) in the cultivated potato [Solanum tuberosum subsp. andigena, line 2x(v-2)7] is located on chromosome XI in a segment that contains three other known resistance genes in other syntenic solanaceous species. One of them is the gene N that controls resistance to tobacco mosaic tobamovirus in tobacco and has previously been isolated and sequenced. Three sequence-related, resistance gene-like (RGL) DNA fragments (354–369 bp) highly homologous to the gene N were PCR-amplified from the potato line 2x(v-2)7. Two RGL fragments (79 and 81% homologous to the N gene) co-segregated with Ry adg among the 77 F1 progeny tested. These RGLs may originate from a resistance gene family on chromosome XI. The potato line 2x(v-2)7 also expressed resistance to potato A potyvirus (PVA), which was controlled by another locus on chromosome XI mapped ca. 6.8 cM distal to Ry adg . Received: 18 December 1997 / Accepted: 30 December 1997 |
| Keywords: | Potato virus A Potato virus Y Resistance gene RFLP Diploid potato |
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