Average quantities accessible to the analysis of sedimentation equilibrium data for self-association systems |
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| Authors: | E Bucci |
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| Affiliation: | 1. Division of Physical Oceanography and Climate Research, University of Gdańsk, Aleja Piłsudskiego 46, 81-378 Gdynia, Poland;2. Institute of Oceanology, Polish Academy of Sciences, Powstańców Warszawy 55, 81-712 Sopot, Poland;3. Division of Geophysics, University of Gdańsk, Aleja Piłsudskiego 46, 81-378 Gdynia, Poland;1. CESAM - Centre for Environmental and Marine Studies & Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;2. Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada;1. Faculty of Forestry, 2424 Main Mall, University of British Columbia, Vancouver, BC V6T 1Z4, Canada;2. Environment & Climate Change Canada, Pacific Wildlife Research Centre, 5421 Robertson Road, Delta, BC V4K 3N2, Canada;3. Department of Earth, Ocean, and Atmospheric Sciences, University of British Columbia, 2207 Main Mall #2020, Vancouver, BC V6T 1Z4, Canada;4. Institute for the Oceans and Fisheries, University of British Columbia, Vancouver, British Columbia, Canada |
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| Abstract: | This paper shows that analysis of sedimentation equilibrium data, searching for average molecular weights, gives quantities which are dependent on the total protein concentration of the samples, while knowledge of the molecular weight of the monomeric subunits allows a more meaningful search for the concentrations of the individual polymeric components of the system. From these the various average molecular weights can be construed, and the various dissociation equilibrium constants evaluated. Also, in this paper considerations are proposed on the meaning of nonideality terms in associating systems and possible ways for estimating them. As an example the proposed procedures have been applied to measurements of sedimentation equilibrium in carbonmonoxyhemoglobin. |
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