A procedure for the estimation over time of metabolic fluxes in scenarios where measurements are uncertain and/or insufficient |
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Authors: | Francisco Llaneras Jesús Picó |
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Institution: | (1) Dept. of Systems Engineering and Control, Technical University of Valencia, Camino de Vera s/n, 46022 Valencia, Spain |
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Abstract: | Background An indirect approach is usually used to estimate the metabolic fluxes of an organism: couple the available measurements with
known biological constraints (e.g. stoichiometry). Typically this estimation is done under a static point of view. Therefore,
the fluxes so obtained are only valid while the environmental conditions and the cell state remain stable. However, estimating
the evolution over time of the metabolic fluxes is valuable to investigate the dynamic behaviour of an organism and also to
monitor industrial processes. Although Metabolic Flux Analysis can be successively applied with this aim, this approach has
two drawbacks: i) sometimes it cannot be used because there is a lack of measurable fluxes, and ii) the uncertainty of experimental
measurements cannot be considered. The Flux Balance Analysis could be used instead, but the assumption of optimal behaviour
of the organism brings other difficulties. |
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Keywords: | |
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