Directed evolution of a filamentous fungus for thermotolerance |
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Authors: | Eudes de Crecy Stefan Jaronski Benjamin Lyons Thomas J Lyons and Nemat O Keyhani |
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Institution: | (1) Evolugate LLC, 2153 SE Hawthorne Road, 15 Gainesville, FL 32641, USA;(2) USDA ARS NPARL, 1500 N. Central Ave., Sidney, MT 59270, USA;(3) Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA |
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Abstract: | Background Filamentous fungi are the most widely used eukaryotic biocatalysts in industrial and chemical applications. Consequently,
there is tremendous interest in methodology that can use the power of genetics to develop strains with improved performance.
For example, Metarhizium anisopliae is a broad host range entomopathogenic fungus currently under intensive investigation as a biologically based alternative
to chemical pesticides. However, it use is limited by the relatively low tolerance of this species to abiotic stresses such
as heat, with most strains displaying little to no growth between 35–37°C. In this study, we used a newly developed automated
continuous culture method called the Evolugator™, which takes advantage of a natural selection-adaptation strategy, to select
for thermotolerant variants of M. anisopliae strain 2575 displaying robust growth at 37°C. |
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