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Non-antibiotic selection systems for soybean somatic embryos: the lysine analog aminoethyl-cysteine as a selection agent
Authors:Suryadevara S Rao  Lewamy Mamadou  Matt McConnell  Raghuveer Polisetty  Prachuab Kwanyuen  David Hildebrand
Institution:(1) Plant & Soil Sciences, University of Kentucky, Lexington, KY 40546, USA;(2) USDA-ARS/Crop Sciences, NC State University Raleigh, NC 27607, USA
Abstract:

Background  

In soybean somatic embryo transformation, the standard selection agent currently used is hygromycin. It may be preferable to avoid use of antibiotic resistance genes in foods. The objective of these experiments was to develop a selection system for producing transgenic soybean somatic embryos without the use of antibiotics such as hygromycin.
Keywords:
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