Metabolic engineering of lactic acid bacteria for the production of nutraceuticals |
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Authors: | Hugenholtz Jeroen Sybesma Wilbert Groot Masja Nierop Wisselink Wouter Ladero Victor Burgess Kay van Sinderen Douwe Piard Jean-Christophe Eggink Gerrit Smid Eddy J Savoy Graciela Sesma Fernando Jansen Tanja Hols Pascal Kleerebezem Michiel |
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Affiliation: | (1) Wageningen Centre for Food Sciences, the Netherlands;(2) NIZO Food Research, Ede, The Netherlands;(3) ATO-DLO, Wageningen, pThe Netherlands;(4) Universite Catholic de Louvain-la-Neuve, Belgium;(5) University College Cork, Ireland;(6) INRA Jouy-en Josas, France;(7) CERELA, Tucuman, Argentina |
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Abstract: | Lactic acid bacteria display a relatively simple and well-described metabolism where the sugar source is converted mainly to lactic acid. Here we will shortly describe metabolic engineering strategies on the level of sugar metabolism, that lead to either the efficient re-routing of the lactococcal sugar metabolism to nutritional end-products other than lactic acid such as L-alanine, several low-calorie sugars and oligosaccharides or to enhancement of sugar metabolism for complete removal of (undesirable) sugars from food materials. Moreover, we will review current metabolic engineering approaches that aim at increasing the flux through complex biosynthetic pathways, leading to the production of the B-vitamins folate and riboflavin. An overview of these metabolic engineering activities can be found on the website of the Nutra Cells 5th Framework EU-project (www.nutracells.com). Finally, the impact of the developments in the area of genomics and corresponding high-throughput technologies on nutraceutical production will be discussed. |
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Keywords: | folate galactose lactic acid bacteria Lactococcus lactis low-calorie sugars mannitol metabolic engineering nutraceuticals oligosaccharides riboflavin sorbitol tagatose |
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