Crosslinking of tropomyosin to myosin subfragment-1 in reconstituted rabbit skeletal thin filaments |
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| Authors: | T Tao M Lamkin |
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| Affiliation: | Department of Muscle Research, Boston Biomedical Research Institute, 20 Staniford Street, Boston MA 02114, USA;Department of Neurology, Harvard Medical School, Boston, MA, USA |
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| Abstract: | Rabbit skeletal tropomyosin was labeled with the bifunctional photoactivatable crosslinker N-succinimidyl-6- (4'-azido-2'-nitrophenylamino)hexanoate. After irradiating the rigor complex composed of myosin subfragment-1, crosslinker-labeled tropomyosin, and F-actin, a crosslinked product was formed. This product was identified as a 1:1 adduct of tropomyosin and subfragment-1. This finding is in support of recent structural studies which suggest that tropomyosin and subfragment-1 are in close proximity to each other, and may be relevant to the mechanism of thin filament regulation. |
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| Keywords: | Skeletal muscle Tropomyosin Myosin Photocrosslinking Tm, rabbit skeletal tropomyosin S1, chymotryptic myosin subfragment-1 ANPH-Tm, Suc-ANPH-labeled Tm |
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