Characterization of the mechanisms involved in the gastric antisecretory effect of TLQP-21, a vgf-derived peptide,in rats |
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Authors: | Valeria Sibilia Francesca Pagani Ilaria Bulgarelli Giovanni Tulipano Roberta Possenti Francesca Guidobono |
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Affiliation: | 1.Department of Pharmacology, Chemotherapy and Medical Toxicology,Università degli Studi di Milano,Milan,Italy;2.Division of Pharmacology and Toxicology, Department of Biomedical Sciences and Biotechnology,Università degli Studi di Brescia,Brescia,Italy;3.Institute of Neurobiology and Molecular Medicine, CNR,Rome,Italy;4.Department of Neuroscience,University “Tor Vergata”,Rome,Italy |
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Abstract: | TLQP-21, a vgf-derived peptide modulates gastric emptying and prevents ethanol-induced gastric lesions in rats. However, it remains to be studied whether or not TLQP-21 affects gastric acid secretion. In this study, we evaluated the effects of central (0.8–8 nmol/rat) or peripheral (48–240 nmol/kg, intraperitoneally) TLQP-21 administration on gastric acid secretion in pylorus-ligated rats. The mechanisms involved in such activity were also examined. Central TLQP-21 injection significantly reduced gastric acid volume and dose-dependently inhibited total acid output (ED50 = 2.71 nmol), while peripheral TLQP-21 administration had no effect. The TLQP-21 antisecretory activity was prevented by cysteamine (300 mg/kg, subcutaneously), a depletor of somatostatin, by indomethacin (0.25 mg/rat, intracerebroventricularly), a non-selective cyclooxygenase inhibitor, and by functional ablation of sensory nerves by capsaicin. We conclude that TLQP-21 could be considered a new member of the large group of regulatory peptides affecting gastric acid secretion. The central inhibitory effect of TLQP-21 on gastric acid secretion is mediated by endogenous somatostatin and prostaglandins and requires the integrity of sensory nerve fibres. |
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