两种温度条件下苯酚对铜绿微囊藻大型变种生长的影响

模拟春秋季水温 (2 2℃ )和夏季水温 (3 0℃ )环境 ,用不同浓度的苯酚处理铜绿微囊藻大型变种。结果显示 :正常水体中该藻在 3 0℃比 2 2℃生长快。浓度C≤ 2 0 0 μg/mL的苯酚对它的生长有促进作用 ,而浓度C≥ 40 0 μg/mL的苯酚则抑制其生长 ,高浓度苯酚胁迫下的藻细胞光合速率 ,可溶性蛋白含量下降 ;细胞膜透性增大 ;超氧阴离子 (O 2 )和丙二醛 (MDA)相对含量升高 ;超氧化物歧化酶 (SOD)活性降低 ,藻体自发荧光较对照减弱。表明该藻的生长更适合于夏季高温条件 ,且较高浓度苯酚对其有较强抑制作用。

Effect of phenol on the growth of Microcystic aeruginosa var.major under different temperature conditions

Microcystic aeruginosa var.major was retreated with different concentrations of phenol under the imitated normal temperature(22 ℃)and summer temperature(30 ℃). The result showed it grows faster under 30 ℃ than that of 22 ℃. Phenol whose concentration lower than or equal to 200 μg/mL can accelerate its growth while inhibit it when the concentration was higher than 400 μg/mL. After being retreated with Phenol, the cell's photosynthetic O_2 evolution rate and soluble protein content decreased, the membrane permeability, the relative content of O~~_2, MDA increased, the SOD activity decreased and the intensity of its spontaneous fluorescence under treatment was weaker. All these illustrated Microcystic aeruginosa var. major was adapt to summer temperature, and phenol of high concentration could inhibit its growth seriously.