| 双裂解法提取血清HBV DNA技术的建立 |
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| 引用本文： | 方悦群,王秉瑞. 双裂解法提取血清HBV DNA技术的建立[J]. 微生物学免疫学进展, 1994, 0(2) |
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| 作者姓名： | 方悦群 王秉瑞 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | 为进行ＰＣＲ实验建立了一种两步裂解血清ＨＢＶ提取其ＤＮＡ的方法。该法首先用ＳＤＳ及蛋白酶消化裂解病毒，随后再进行碱裂解，操作较酚提取法大为简化，而ＰＣＲ实验进行的两法的灵敏性比较，说明双裂解法至少不低于酚法。
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| 关 键 词： | DNA提取，乙肝病毒，多聚酶链反应 |
The Technique of Double-Iysis for Serum HBV DNA Extraction |
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| Fang Yuequn,Wang Bingrui. The Technique of Double-Iysis for Serum HBV DNA Extraction[J]. Progress In Microbiology and Immunology, 1994, 0(2) |
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| Authors: | Fang Yuequn Wang Bingrui |
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| Abstract: | A technique of the double-lysis for serum HBV DNA extraction was developed.The virus DNA was firstly disposed of by SDS and proteinase and then by alkali. The method was much simpler than the phenol extraction and its sensiticity was not lower than the latter. 10~-5 dilutions of a HBV serum sample were both determined by the two methods. the double-lysis could cooperate PCR to be applied in the clinical diagnosis. |
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| Keywords: | DNA extraction HBV PCR |
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