| 猪防御素基因PBD-I在大肠杆菌中的重组和融合表达 |
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| 引用本文： | 罗刚,魏泓. 猪防御素基因PBD-I在大肠杆菌中的重组和融合表达[J]. 遗传, 2003, 25(2): 146-150 |
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| 作者姓名： | 罗刚 魏泓 |
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| 作者单位： | 第三军医大学实验动物中心,重庆 400038 |
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| 基金项目： | 国家重点基础研究发展规划项目资助课题(G2000016106) |
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| 摘 要： | 用RT-PCR方法获得PBD-I基因，将该基因插入原核表达载体PinPointTMXa-3中，重组质粒转化大肠杆菌JM109，经IPTG诱导后以融合蛋白形式表达。SDS-PAGE电泳显示PBD-I基因在目标位置17kDa处获得了表达。PBD-I基因的成功表达为进一步研究其抗菌活性、抗菌机理及应用研究形成基础。
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| 关 键 词： | 融合 基因重组 猪防御素 |
| 文章编号： | 0253-9772(2003)02-0146-05 |
| 修稿时间： | 2002-07-05 |
Recombination and Fusion Expression of Porcine Defensin Gene PBD-I in E.coli |
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| Gang Luo,Hong Wei. Recombination and Fusion Expression of Porcine Defensin Gene PBD-I in E.coli[J]. Hereditas, 2003, 25(2): 146-150 |
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| Authors: | Gang Luo Hong Wei |
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| Affiliation: | Laboratory Animal Center,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | The porcine defensin gene PBD-I was amplified by RT-PCR, then the gene was inserted into expression vector PinPoint(TM) Xa-3. Recombinant plasmid named as ppd-1 was transformed into E.Coli JM109, which could effectively produce fusion protein induced with IPTG. The positive clone of PBD-I gene expressed 17kDa fusion protein by SDS-PAGE electrophoresis. Expression of PBD-I gene didn't increase distinctly along with time. The expression of PBD-I gene lays a foundation in research on antimicrobial activities and its mechanism of the defensin. |
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| Keywords: | porcine defensin gene recombination fusion |
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