Biodosimetry estimate for high-LET irradiation |
| |
Authors: | Z Z Wang W J Li D J Zhi X G Jing W Wei Q X Gao B Liu |
| |
Institution: | (1) Laboratory for Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, Gansu Province, China;(2) Graduate School of Chinese Academy of Sciences, Beijing, China;(3) Life Science School of Lanzhou University, Lanzhou, China |
| |
Abstract: | The purpose of this paper is to prepare for an easy and reliable biodosimeter protocol for radiation accidents involving high-linear
energy transfer (LET) exposure. Human peripheral blood lymphocytes were irradiated using carbon ions (LET: 34.6 keV μm−1), and the chromosome aberrations induced were analyzed using both a conventional colcemid block method and a calyculin A
induced premature chromosome condensation (PCC) method. At a lower dose range (0–4 Gy), the measured dicentric (dics) and
centric ring chromosomes (cRings) provided reasonable dose information. At higher doses (8 Gy), however, the frequency of
dics and cRings was not suitable for dose estimation. Instead, we found that the number of Giemsa-stained drug-induced G2
prematurely condensed chromosomes (G2-PCC) can be used for dose estimation, since the total chromosome number (including fragments)
was linearly correlated with radiation dose (r = 0.99). The ratio of the longest and the shortest chromosome length of the drug-induced G2-PCCs increased with radiation
dose in a linear-quadratic manner (r = 0.96), which indicates that this ratio can also be used to estimate radiation doses. Obviously, it is easier to establish
the dose response curve using the PCC technique than using the conventional metaphase chromosome method. It is assumed that
combining the ratio of the longest and the shortest chromosome length with analysis of the total chromosome number might be
a valuable tool for rapid and precise dose estimation for victims of radiation accidents. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|