High-frequency oscillations of phrenic activity in eupnea and gasping of in situ rat: influence of temperature

We hypothesized that the in situ perfused preparation of the juvenile rat exhibits patterns of ventilatory activity comparable to eupnea and gasping in vivo. To evaluate this hypothesis, we examined high-frequency oscillations of activity of the phrenic nerve at 27-34 degrees C. The peak frequency of these high-frequency oscillations was defined from power spectral analysis. In situ, recordings were obtained in hyperoxic normocapnia, during ventilatory cycles in which the peak of integrated phrenic activity was achieved late in the burst, as in eupnea in vivo. Recordings were also obtained in hypoxic hypercapnia, when the peak of integrated phrenic activity occurred in the first half of the burst, as in gasping in vivo. In situ, peak frequencies in the power spectra were significantly higher in gasping than during eupnea. Frequencies during eupnea and gasping were progressively elevated as the temperature of the in situ preparation was increased. The shift in peak frequencies between eupnea and gasping and the temperature sensitivity of frequencies in situ were the same as in vivo. Results provide additional support for the conclusion that the in situ preparation demonstrates distinctly different patterns of automatic ventilatory activity, comparable to eupnea and gasping in vivo.