Biofilm Formation and Biocide Susceptibility Testing of Mycobacterium fortuitum and Mycobacterium marinum |
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| Authors: | Bardouniotis Elias Ceri Howard Olson Merle E |
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| Institution: | (1) Department of Biological Sciences, University of Calgary, 2500 University Drive N.W., Calgary, Alberta T2N 1N4, Canada, CA;(2) Biofilm Research Group, University of Calgary, 2500 University Drive N.W., Calgary, Alberta T2N 1N4, Canada, CA;(3) Microbiology and Infectious Diseases, University of Calgary, Health Sciences Centre, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada, CA |
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| Abstract: | The ability of non-tuberculous mycobacteria to form biofilms may allow for their increased resistance to currently used biocides
in medical and industrial settings. This study examines the biofilm growth of Mycobacterium fortuitum and Mycobacterium marinum, using the MBEC™ assay system, and compares the susceptibility of planktonic and biofilm cells to commercially available
biocides. With scanning electron microscopy, both M. fortuitum and M. marinum form biofilms that are morphologically distinct. Biocide susceptibility testing suggested that M. fortuitum biofilms displayed increased resistance over their planktonic state. This is contrasted with M. marinum biofilms, which were generally as or more susceptible over their planktonic state.
Received: 15 February 2002 / Accepted: 28 March 2002 |
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