Protein microarray: A key approach of proteomics

Proteins are vital parts of living organisms, for they consist of the main components of physiological metabolic pathways. The expression and modification status of proteins in cells are time- and space-dependent based upon functions as well as responses to stress. A subject aiming at understanding of protein status under certain physiological condition in a large scale, termed proteomics, is fundamentally important in life science. Although the proteomic investigation mainly relies on electrophoresis, liquid chromatography, and mass spectrometry, protein microarray is emerging as one of new techniques, and its power is being recognized in the field recently. Herein, we highlight progress in protein microarray, both its generation and application.