In vitro binding of the CcpA protein of Bacillus megaterium to cis-acting catabolite responsive elements (CREs) of Gram-positive bacteria |
| |
Authors: | Tom M. Ramseier,Jonathan Reizer,Elke Kü ster,Wolfgang Hillen,Milton H. Saier |
| |
Affiliation: | Department of Biology, University of California at San Diego, La Jolla, CA 92093-0116, USA; Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik der Friedrich-Alexander Universität Erlangen-Nürnberg, 91058 Erlangen, Germany |
| |
Abstract: | Abstract Using DNA band migration retardation assays, specific binding of the CcpA protein of Bacillus megaterium to the ds-acting catabolite responsive element (CRE) of the xyl operon of B. subtilis has been demonstrated. Binding of CcpA was specifically inhibited by addition of unlabeled DNA fragments containing CREs of other operons but not by DNA fragments lacking a CRE. Binding was stimulated by high concentrations of phosphate, pyrophosphate, and organic phosphate esters and specifically inhibited by serine phosphorylated HPr and its conformational analogue, S46D HPr. This report therefore documents the specific binding of CcpA to a target CRE and defines its regulation by HPr(ser-P) and phosphorylated metabolites. |
| |
Keywords: | Catabolite repression Catabolite responsive element (CRE) CcpA Gram-positive bacteria In vitro DNA binding Xylose (xyl) operon Bacillus megaterium |
|
|