甲状腺素和甲巯咪唑对大鼠视前区-下丘脑前部中及血清中单胺类递质的影响

反复皮下注射甲状腺素(T_4,1mg/100g·d,连续10d)和胃饲甲巯咪唑(10mg/100g·d,连续15d)后,用高效液相色谱加电化学检测器(HPLC-EGD)分析了大鼠视前区-下丘脑前部(PO/AH)中单胺神经递质的含量。发现经甲状腺素处理后,PO/AH区中多巴胺(DA)和高香草酸(HVA)含量显著增加,具有统计学意义(P<0.01),5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)轻度上升,但无统计学意义(P>0.05),去甲肾上腺素(NE)无明显变化;经甲巯咪唑处理后的动物则出现NE含量显著下降(P<0.05),但DA、HVA、5-HT和5-HIAA各项变化不显著。本文还同步观察了外周血清中单胺介质含量的变化,结果表明其与中枢单胺介质含量变化基本上不同步。本文还讨论了甲状腺素和甲巯咪唑影响单胺介质含量和影响体温变化之间的关系。

EFFECTS OF THYROXINE AND METHIMAZOLUM ON MONAMINE TRANSMITTERS IN PREOPTIC/ANTERIOR HYPOTHALAMIC AREA AND PERIPHERAL SERUM IN RATS

The contents of monamine transmitters in preoptic /arterior hypothalamic(PO / AH) area and in the serum of rats injected with thyroxine (T_4, 1 mg /100 gbody wt/d, for 10 d, hypodermic i.) and fed with methimazolum (10 mg/ 100 gbody wt /d, for 15 d) were assayed by high pressure liquid chromatography withelectrochemical detector (HPLC-ECD). It was found that the content of dopa-mine and homovanillic acid in PO /AH area showed significant incresae (P<0.01) associated with a slight rise (P>05) of 5-hydroxytryptamine (5-HT) and5-hydroxyindoleacetic acid (5-HIAA), and with no change of norepinephrine inthe thyroxine group. After fed with methimazolum, the conent of norepine-phrine decreased significantly (P<0.05), but no obvious changes occurred indopamine, homovanillic acid, 5-HT and 5-HIAA. By synchronous analysingof monamine transmitters in peripheral serum, it was showed that there was no sig-nificant linear relationship between the changes of monamine transmitters in thebrain and in the serum. The correlation between thyroxine and methimazolumon the content of monamine transmitters and on the change of body temperaturewas also discussed.