Analysis of the 5′ non-coding region of rat liver S-adenosylmethionine synthetase mRNA and comparison of the Mr deduced from the cDNA sequence and the purified enzyme |
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| Authors: | Luis Alvarez Miryam Asuncin Fernando Corrales Maria A Pajares Jos M Mato |
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| Institution: | Luis Alvarez, Miryam Asunción, Fernando Corrales, Maria A. Pajares,José M. Mato |
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| Abstract: | A 3 kb cDNA coding for rat liver S-adenosylmethionine (AdoMet) synthetase has been isolated. The Mr of the protein has been unequivocally determined by cDNA sequencing and enzyme purification on a thiopropyl-Sepharose column. The length of the mRNA 5′ non-coding region has been defined by primer-extension analysis. The rat liver cloned cDNA has been also used to detect S-adenosylmethionine synthetase mRNA in human liver. |
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| Keywords: | S-Adenosylmethionine synthetase cDNA sequence Sulfhydryl groups Messenger RNA Rat liver Ruman liver |
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