Infra-red spectroscopic study for decalcification in the dentine |
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Authors: | Ken Fukuda |
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Affiliation: | (1) Department of Oral Surgery, Osaka University Dental School, Osaka, Japan |
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Abstract: | Summary A study was made of spectra of decalcified human dentine being treated with five decalcifying agents to investigate the differences of infrared spectra of decalcified dentine.Solutions of 20% 4-Na-EDTA, 5% HNO3, 10% sulfosalicylic acid, 10% CCl3COOH and 10% lithium lactate were used as decalcifying agents. Infrared absorption spectra between 1800 and 650 cm–1 were obtained for the investigation of the absorption strength at 1033,1095; 1412–1414 to 1450–1455; 875–870; 1649 and 1550 cm–1 before and after decalcification.The specimens following decalcification with EDTA and HNO3 showed almost no influences to the absorption strength at 1649 and 1550 cm–1 of amides. Such absorption spectra were relatively slight in CCl3COOH and sulfosalicylic acid. The absorption strength at 1033 and 1095 cm–1 bands of PO4–––may indicate a standard of decalcification. Quick and complete decalcification of dentine was performed by HNO3, CCl3COOH and sulfosalicylic acid but slower and more incomplete decalcification resulted with EDTA and lithium lactate. |
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