A cytoplasmic cAMP-binding protein in Dictyostelium discoideum |
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| Authors: | B H Leichtling C Tihon E Spitz H V Rickenberg |
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| Institution: | 1. Department of Molecular and Cellular Biology, National Jewish Hospital and Research Center, Denver, Colorado 80206 USA;1. Department of Biochemistry, Biophysics and Genetics, University of Colorado School of Medicine, Denver, Colorado 80262 USA |
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| Abstract: | A cytoplasmic cAMP-binding protein from Dictyostelium discoideum was purified about 1200-fold. The binding protein is relatively specific for cAMP, but also binds some other adenine derivatives; it has a molecular weight of approximately 185,000 and an apparent KD of 1 μM cAMP. The highest level of cytoplasmic cAMP-binding activity is found in amoebae which have been starved for 0–2 hr. Amoebal extracts contain inhibitors of cAMP binding which are removed by chromatography through Sephacryl S200. |
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