The 5'-termini of the oligonucleotides from ribonuclease T 1 digested E. coli ribosomes |
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Authors: | J C Lee I V Quintanilla |
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Affiliation: | Department of Biochemistry The University of Texas Health Science Center at San Antonio San Antonio, Texas 78284 USA |
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Abstract: | Oligonucleotides remaining in the 70s ribosomal particles after varying degrees of digestion with ribonuclease T1 were phosphorylated with polynucleotide kinase in the presence of γ-labeled32P-ATP. The resulting radioactively labeled RNA molecules were further digested with pancreatic ribonuclease and analyzed by a two-dimensional finger-printing technique. The numbers of labeled oligonucleotides were proportional to the duration of T1 digestion; most of these oligonucleotides yielded 1pAp and/or 1pCp as their 5′-end groups upon alkaline hydrolysis. |
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