A hydroponic culture system for growing<Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> plantlets under sterile conditions |
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Authors: | Bernhard?Schlesier Frédéric?Bréton Email author" target="_blank">Hans-Peter?MockEmail author |
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Institution: | 1.Institute of Plant Genetics and Crop Plant Research,Gatersleben,Germany |
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Abstract: | We developed a hydroponic cultivation system for growingArabidopsis plantlets under sterile, controlled environmental conditions. The system consists of a piece of stainless-steel wire cloth
(125 μm mesh size) that is fixed between 2 flat rings and held in place by 3 legs, placed in a commercially-available glass
jar, and covered by the original glass lid or a sheet of sterilized cellophane. Sterilized seeds were distributed evenly across
the mesh piece, the size of which allowed root growth and kept the seeds in place. After 3 weeks of cultivation, shoot and
root tissues were easily harvested without mechanical damage. Proteome and metabolite analyses were performed on root and
shoot tissues and demonstrated excellent reproducibility, indicating that the system is advantageous when biological variation
is minimized. Induction experiments can be performed by transferring the apparatus (with plants) to a new jar containing a
different nutrient solution. The apparatus is reusable and can easily be sterilized by autoclaving or dry heat. The system
can be adapted to other small-seed plants by varying the mesh size. |
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Keywords: | Arabidopsis metabolic profile proteome roots sterile hydroponic culture |
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