SMB基因与拟南芥细胞脱分化

应用Affymetrix基因芯片技术研究脱分化过程的全基因组表达情况,分析拟南芥叶柄细胞在脱分化过程中的差异表达基因。结果显示:(1)脱分化叶柄有4 222个基因表现出2倍或以上的表达差异,其中1 684个基因表达上调,2 538个基因表达下调。(2)半定量RT-PCR对部分差异表达基因进行验证,进一步筛选出参与拟南芥脱分化候选基因SMB(SOMBRERO)。(3)实时PCR结果表明,野生型叶柄诱导脱分化时,SMB基因表达量随着诱导时间延长而逐渐增加。(4)SMB基因功能缺失突变体smb-3经CIM诱导很难形成愈伤组织;在激素诱导不同时间段,smb-3叶柄没有明显脱分化现象,SMB基因缺失造成拟南芥叶柄脱分化障碍。研究表明,SMB基因参与拟南芥叶柄细胞脱分化过程。

SMB Gene Involved in Cell Dedifferentiation of Arabidopsis thaliana

Different expression genes in the dedifferentiation progress with Affymetrix chips were analyzed.(1)According to data of the chips,there are 4 222 different expression genes,1 684 of them are up-regulated expression and 2 538 genes are down-regulated.(2)The genes relation to cell dedifferentiation according the data of chip and semi-qualitative RT-PCR were further identified and screened,and SMB (SOMBRERO) gene was firstly isolated.(3)SMB gene expression pattern was analyzed with real-time PCR.The gene expression level of SMB increased gradually with the time undergoing cell dedifferentiation.(4)The function of SMB gene undergoing plant cell dedifferentiation was further demonstrated.Test and verify mutant SMB gene in Arabidopsis can affect the procession of cell dedifferentiation and the ability of forming callus.This research demonstrated that SMB gene involves in cell dedifferentiation of Arabidopsis thaliana.